Mark Mimee

Assistant Professor
Websites
Research Summary
The Mimee Lab leverages synthetic biology to engineer the microbiome, exploring strategies to modify commensal bacteria and bacteriophage for diagnostic and therapeutic applications.By genetically manipulating commensal microbes, we seek to create living devices that can serve as biosensors to probe the structure and function of the microbiome and as cell-based therapeutics for infectious and inflammatory disease. Additionally, we develop approaches to augment the natural properties of viruses that infect bacteria, called bacteriophage, to create novel therapies for antibiotic-resistant bacterial infections. Our long-term vision is to implement these synthetic biology technologies to chart new basic and translational studies to exploit the microbiota for human health. https://voices.uchicago.edu/mimeelab/
Keywords
Microbiota, Synthetic Biology, Bioengineering, Bacteriophage, Host-Pathogen Interactions
Education
  • Massachusetts Insitute of Technology, Cambridge, MA, USA, Synthetic Biology 08/2019
  • Massachusetts Insitute of Technology, Cambridge, MA, USA, PhD Microbiology 06/2018
  • McGill University, Montreal, QC, Canada, BSc Microbiology & Immunology 06/2011
Biosciences Graduate Program Association
Publications
  1. McClure S, Enam F, Arnold J, Mimee M. Proceedings from the 3rd International Conference on Microbiome Engineering. Biotechnol Prog. 2022 05; 38(3):e3241. View in: PubMed

  2. Mimee M, Nagler CR. Engineered yeast tune down gut inflammation. Nat Med. 2021 Jul; 27(7):1150-1151. View in: PubMed

  3. Yehl K, Lemire S, Yang AC, Ando H, Mimee M, Torres MT, de la Fuente-Nunez C, Lu TK. Engineering Phage Host-Range and Suppressing Bacterial Resistance through Phage Tail Fiber Mutagenesis. Cell. 2019 10 03; 179(2):459-469.e9. View in: PubMed

  4. Inda ME, Mimee M, Lu TK. Cell-based biosensors for immunology, inflammation, and allergy. J Allergy Clin Immunol. 2019 09; 144(3):645-647. View in: PubMed

  5. Mimee M, Nadeau P, Hayward A, Carim S, Flanagan S, Jerger L, Collins J, McDonnell S, Swartwout R, Citorik RJ, Bulovic V, Langer R, Traverso G, Chandrakasan AP, Lu TK. An ingestible bacterial-electronic system to monitor gastrointestinal health. Science. 2018 05 25; 360(6391):915-918. View in: PubMed

  6. Mimee M, Citorik RJ, Lu TK. Microbiome therapeutics - Advances and challenges. Adv Drug Deliv Rev. 2016 10 01; 105(Pt A):44-54. View in: PubMed

  7. Mimee M, Tucker AC, Voigt CA, Lu TK. Programming a Human Commensal Bacterium, Bacteroides thetaiotaomicron, to Sense and Respond to Stimuli in the Murine Gut Microbiota. Cell Syst. 2016 Mar 23; 2(3):214. View in: PubMed

  8. Mimee M, Tucker AC, Voigt CA, Lu TK. Programming a Human Commensal Bacterium, Bacteroides thetaiotaomicron, to Sense and Respond to Stimuli in the Murine Gut Microbiota. Cell Syst. 2015 Jul 29; 1(1):62-71. View in: PubMed

  9. Citorik RJ, Mimee M, Lu TK. Sequence-specific antimicrobials using efficiently delivered RNA-guided nucleases. Nat Biotechnol. 2014 Nov; 32(11):1141-5. View in: PubMed

  10. Citorik RJ, Mimee M, Lu TK. Bacteriophage-based synthetic biology for the study of infectious diseases. Curr Opin Microbiol. 2014 Jun; 19:59-69. View in: PubMed

  11. Thanabalasuriar A, Bergeron J, Gillingham A, Mimee M, Thomassin JL, Strynadka N, Kim J, Gruenheid S. Sec24 interaction is essential for localization and virulence-associated function of the bacterial effector protein NleA. Cell Microbiol. 2012 Aug; 14(8):1206-18. View in: PubMed

  12. Thanabalasuriar A, Koutsouris A, Weflen A, Mimee M, Hecht G, Gruenheid S. The bacterial virulence factor NleA is required for the disruption of intestinal tight junctions by enteropathogenic Escherichia coli. Cell Microbiol. 2010 Jan; 12(1):31-41. View in: PubMed