Dominique Missiakas

Research Summary
Dr. Missiakas’s research program examines the Gram-positive pathogens Staphylococcus aureus and Bacillus anthracis. This program seeks to appreciate the biogenesis of the envelope of these microorganisms as well as the secretion of their virulence factors and host pathways targeted by these pathogens. Pathogenesis and vaccine studies in S. aureus: Many molecules in the envelope of these bacteria affect their interactions with the host. In staphylococci, cell wall anchored proteins contribute to pathogen survival in blood, dissemination to tissues, formation of abscesses and persistence in host tissues as well as subversion of immune functions. The systematic analysis of mutants lacking all or anyone of twenty cell wall anchored proteins has proven a very powerful approach in identifying protective antigens for vaccine design and elucidating virulence pathways. S. aureus is the only bacterial pathogen known to coagulate plasma and to agglutinate with fibrin cables in blood. A molecular examination of agglutination revealed that this process requires the secreted products of the coa (coagulase), vwb (von Willebrand binding protein), and clfA (Clumping factor A) genes. We are examining a model whereby S. aureus agglutination involves the formation of staphylothrombin (Coa, vWbp)-assembled fibrin cables, capped by clumping factor A (ClfA) on the staphylococcal surface and crosslinked by factor XIII. In this model, S. aureus agglutination provides for escape from phagocytic killing and bacterial traffic across the vasculature. In this manner, the Coa-vWbp-ClfA mediated agglutination pathway promotes the formation of abscess lesions, where S. aureus replicates as a fibrin encapsulated bacterial community, protected from immune cells. This key virulence strategy can be perturbed with monoclonal antibodies and small molecule inhibitors to either prevent or treat S. aureus sepsis Cell envelope assembly: Bacteria assemble polymers in their envelope that are important for their viability as well as their interaction with hosts. Our research program combines bacterial genetics, physiology and biochemistry as well as small molecule chemical tools approaches to characterize the complex biochemical pathways that enable envelope assembly in Gram-positive microbes. Potential innovations derived from this work are the identification of key enzymes for the biosynthesis of lipoteichoic acid (LTA), wall teichoic acid (WTA), secondary cell wall polysaccharide (SCWP) and capsular polymer (CPS, PDGA). In Bacilli, the SCWP is also involved in the retention of proteins that bear the S-layer homology domain (SLH proteins). SLH proteins play important function for cell separation, sporulation as well as virulence. Protein secretion: One aspect of this program examines a Sec-independent secretion system named ESAT-6 like Secretion System (ESS). Biochemical and genetic approaches are used to isolate and characterize the ESS translocon, identify secreted substrates and elucidate their function during infection of hosts.
Bacterial pathogenesis, Bacterial genetics, Bacterial physiology, Envelope assembly, Protein secretion, Vaccine
  • Université of Paris XI, Orsay, France, Ph.D. Biochemistry 1991
Biosciences Graduate Program Association
  1. Malachowa N, McGuinness W, Kobayashi SD, Porter AR, Shaia C, Lovaglio J, Smith B, Rungelrath V, Saturday G, Scott DP, Falugi F, Missiakas D, Schneewind O, DeLeo FR. Toward Optimization of a Rabbit Model of Staphylococcus aureus (USA300) Skin and Soft Tissue Infection. Microbiol Spectr. 2022 04 27; 10(2):e0271621. View in: PubMed

  2. Teplensky MH, Distler ME, Kusmierz CD, Evangelopoulos M, Gula H, Elli D, Tomatsidou A, Nicolaescu V, Gelarden I, Yeldandi A, Batlle D, Missiakas D, Mirkin CA. Spherical nucleic acids as an infectious disease vaccine platform. Proc Natl Acad Sci U S A. 2022 04 05; 119(14):e2119093119. View in: PubMed

  3. Hassler L, Wysocki J, Gelarden I, Sharma I, Tomatsidou A, Ye M, Gula H, Nicoleascu V, Randall G, Pshenychnyi S, Khurram N, Kanwar Y, Missiakas D, Henkin J, Yeldandi A, Batlle D. A Novel Soluble ACE2 Protein Provides Lung and Kidney Protection in Mice Susceptible to Lethal SARS-CoV-2 Infection. J Am Soc Nephrol. 2022 Jul; 33(7):1293-1307. View in: PubMed

  4. El-Shennawy L, Hoffmann AD, Dashzeveg NK, McAndrews KM, Mehl PJ, Cornish D, Yu Z, Tokars VL, Nicolaescu V, Tomatsidou A, Mao C, Felicelli CJ, Tsai CF, Ostiguin C, Jia Y, Li L, Furlong K, Wysocki J, Luo X, Ruivo CF, Batlle D, Hope TJ, Shen Y, Chae YK, Zhang H, LeBleu VS, Shi T, Swaminathan S, Luo Y, Missiakas D, Randall GC, Demonbreun AR, Ison MG, Kalluri R, Fang D, Liu H. Circulating ACE2-expressing extracellular vesicles block broad strains of SARS-CoV-2. Nat Commun. 2022 01 20; 13(1):405. View in: PubMed

  5. Chen X, Schneewind O, Missiakas D. Engineered human antibodies for the opsonization and killing of Staphylococcus aureus. Proc Natl Acad Sci U S A. 2022 01 25; 119(4). View in: PubMed

  6. Chen X, Sun Y, Missiakas D, Schneewind O. Corrigendum to: Staphylococcus aureus Decolonization of Mice With Monoclonal Antibody Neutralizing Protein A. J Infect Dis. 2022 Jan 18; 225(2):358. View in: PubMed

  7. Cai X, Chen M, Prominski A, Lin Y, Ankenbruck N, Rosenberg J, Nguyen M, Shi J, Tomatsidou A, Randall G, Missiakas D, Fung J, Chang EB, Penaloza-MacMaster P, Tian B, Huang J. A Multifunctional Neutralizing Antibody-Conjugated Nanoparticle Inhibits and Inactivates SARS-CoV-2. Adv Sci (Weinh). 2022 01; 9(2):e2103240. View in: PubMed

  8. Drayman N, DeMarco JK, Jones KA, Azizi SA, Froggatt HM, Tan K, Maltseva NI, Chen S, Nicolaescu V, Dvorkin S, Furlong K, Kathayat RS, Firpo MR, Mastrodomenico V, Bruce EA, Schmidt MM, Jedrzejczak R, Muñoz-Alía MÁ, Schuster B, Nair V, Han KY, O'Brien A, Tomatsidou A, Meyer B, Vignuzzi M, Missiakas D, Botten JW, Brooke CB, Lee H, Baker SC, Mounce BC, Heaton NS, Severson WE, Palmer KE, Dickinson BC, Joachimiak A, Randall G, Tay S. Masitinib is a broad coronavirus 3CL inhibitor that blocks replication of SARS-CoV-2. Science. 2021 08 20; 373(6557):931-936. View in: PubMed

  9. Shi M, Chen X, Sun Y, Kim HK, Schneewind O, Missiakas D. A protein A based Staphylococcus aureus vaccine with improved safety. Vaccine. 2021 06 29; 39(29):3907-3915. View in: PubMed

  10. Shi M, Willing SE, Kim HK, Schneewind O, Missiakas D. Peptidoglycan Contribution to the B Cell Superantigen Activity of Staphylococcal Protein A. mBio. 2021 04 20; 12(2). View in: PubMed

  11. Viljoen A, Viela F, Mathelié-Guinlet M, Missiakas D, Pietrocola G, Speziale P, Dufrêne YF. Staphylococcus aureus vWF-binding protein triggers a strong interaction between clumping factor A and host vWF. Commun Biol. 2021 04 12; 4(1):453. View in: PubMed

  12. Willing S, Schneewind O, Missiakas D. Regulated cleavage of glycan strands by the murein hydrolase SagB in S. aureus involves a direct interaction with LyrA (SpdC). J Bacteriol. 2021 Feb 16. View in: PubMed

  13. Missiakas D, Winstel V. Selective Host Cell Death by Staphylococcus aureus: A Strategy for Bacterial Persistence. Front Immunol. 2020; 11:621733. View in: PubMed

  14. Na M, Hu Z, Mohammad M, Stroparo MDN, Ali A, Fei Y, Jarneborn A, Verhamme P, Schneewind O, Missiakas D, Jin T. The Expression of von Willebrand Factor-Binding Protein Determines Joint-Invading Capacity of Staphylococcus aureus, a Core Mechanism of Septic Arthritis. mBio. 2020 11 17; 11(6). View in: PubMed

  15. Chen X, Shi M, Tong X, Kim HK, Wang LX, Schneewind O, Missiakas D. Glycosylation-dependent opsonophagocytic activity of staphylococcal protein A antibodies. Proc Natl Acad Sci U S A. 2020 09 15; 117(37):22992-23000. View in: PubMed

  16. Willing S, Dyer E, Schneewind O, Missiakas D. FmhA and FmhC of Staphylococcus aureus incorporate serine residues into peptidoglycan cross-bridges. J Biol Chem. 2020 09 25; 295(39):13664-13676. View in: PubMed

  17. Chateau A, Oh SY, Tomatsidou A, Brockhausen I, Schneewind O, Missiakas D. Distinct Pathways Carry Out a and ß Galactosylation of Secondary Cell Wall Polysaccharide in Bacillus anthracis. J Bacteriol. 2020 07 09; 202(15). View in: PubMed

  18. Chun LY, Dolle-Molle L, Bethel C, Dimitroyannis RC, Williams BL, Schechet SA, Hariprasad SM, Missiakas D, Schneewind O, Beavis KG, Skondra D. Rapid pathogen identification and antimicrobial susceptibility testing in in vitro endophthalmitis with matrix assisted laser desorption-ionization Time-of-Flight Mass Spectrometry and VITEK 2 without prior culture. PLoS One. 2019; 14(12):e0227071. View in: PubMed

  19. Winstel V, Schneewind O, Missiakas D. Staphylococcus aureus Exploits the Host Apoptotic Pathway To Persist during Infection. mBio. 2019 11 12; 10(6). View in: PubMed

  20. Osei-Owusu P, Charlton TM, Kim HK, Missiakas D, Schneewind O. FPR1 is the plague receptor on host immune cells. Nature. 2019 10; 574(7776):57-62. View in: PubMed

  21. Kim HK, Premaratna R, Missiakas DM, Schneewind O. Rickettsia conorii O antigen is the target of bactericidal Weil-Felix antibodies. Proc Natl Acad Sci U S A. 2019 09 24; 116(39):19659-19664. View in: PubMed

  22. Schneewind O, Missiakas DM. Staphylococcal Protein Secretion and Envelope Assembly. Microbiol Spectr. 2019 07; 7(4). View in: PubMed

  23. Liesenborghs L, Meyers S, Lox M, Criel M, Claes J, Peetermans M, Trenson S, Vande Velde G, Vanden Berghe P, Baatsen P, Missiakas D, Schneewind O, Peetermans WE, Hoylaerts MF, Vanassche T, Verhamme P. Staphylococcus aureus endocarditis: distinct mechanisms of bacterial adhesion to damaged and inflamed heart valves. Eur Heart J. 2019 10 14; 40(39):3248-3259. View in: PubMed

  24. Chateau A, Schneewind O, Missiakas D. Extraction and Purification of Wall-Bound Polymers of Gram-Positive Bacteria. Methods Mol Biol. 2019; 1954:47-57. View in: PubMed

  25. Putra I, Rabiee B, Anwar KN, Gidfar S, Shen X, Babalooee M, Ghassemi M, Afsharkhamseh N, Bakhsh S, Missiakas D, Nezamabadi A, Milani B, Eslani M, Djalilian AR. Staphylococcus aureus alpha-hemolysin impairs corneal epithelial wound healing and promotes intracellular bacterial invasion. Exp Eye Res. 2019 04; 181:263-270. View in: PubMed

  26. Missiakas D. Staphylococcus aureus TarP: A Brick in the Wall or Rosetta Stone? Cell Host Microbe. 2019 02 13; 25(2):182-183. View in: PubMed

  27. Schneewind O, Missiakas D. Sortases, Surface Proteins, and Their Roles in Staphylococcus aureus Disease and Vaccine Development. Microbiol Spectr. 2019 01; 7(1). View in: PubMed

  28. Chen X, Sun Y, Missiakas D, Schneewind O. Staphylococcus aureus Decolonization of Mice With Monoclonal Antibody Neutralizing Protein A. J Infect Dis. 2019 02 23; 219(6):884-888. View in: PubMed

  29. Mancini S, Oechslin F, Menzi C, Que YA, Claes J, Heying R, Veloso TR, Vanassche T, Missiakas D, Schneewind O, Moreillon P, Entenza JM. Marginal role of von Willebrand factor-binding protein and coagulase in the initiation of endocarditis in rats with catheter-induced aortic vegetations. Virulence. 2018; 9(1):1615-1624. View in: PubMed

  30. Bobrovskyy M, Willing SE, Schneewind O, Missiakas D. EssH Peptidoglycan Hydrolase Enables Staphylococcus aureus Type VII Secretion across the Bacterial Cell Wall Envelope. J Bacteriol. 2018 10 15; 200(20). View in: PubMed

  31. Pigozzo AB, Missiakas D, Alonso S, Dos Santos RW, Lobosco M. Development of a Computational Model of Abscess Formation. Front Microbiol. 2018; 9:1355. View in: PubMed

  32. Winstel V, Missiakas D, Schneewind O. Staphylococcus aureus targets the purine salvage pathway to kill phagocytes. Proc Natl Acad Sci U S A. 2018 06 26; 115(26):6846-6851. View in: PubMed

  33. Yu W, Missiakas D, Schneewind O. Septal secretion of protein A in Staphylococcus aureus requires SecA and lipoteichoic acid synthesis. Elife. 2018 05 14; 7. View in: PubMed

  34. Ahmed MM, Aboshanab KM, Ragab YM, Missiakas DM, Aly KA. The transmembrane domain of the Staphylococcus aureus ESAT-6 component EssB mediates interaction with the integral membrane protein EsaA, facilitating partially regulated secretion in a heterologous host. Arch Microbiol. 2018 Sep; 200(7):1075-1086. View in: PubMed

  35. Sun Y, Emolo C, Holtfreter S, Wiles S, Kreiswirth B, Missiakas D, Schneewind O. Staphylococcal Protein A Contributes to Persistent Colonization of Mice with Staphylococcus aureus. J Bacteriol. 2018 05 01; 200(9). View in: PubMed

  36. Chateau A, Lunderberg JM, Oh SY, Abshire T, Friedlander A, Quinn CP, Missiakas DM, Schneewind O. Galactosylation of the Secondary Cell Wall Polysaccharide of Bacillus anthracis and Its Contribution to Anthrax Pathogenesis. J Bacteriol. 2018 03 01; 200(5). View in: PubMed

  37. Aly KA, Anderson M, Ohr RJ, Missiakas D. Isolation of a Membrane Protein Complex for Type VII Secretion in Staphylococcus aureus. J Bacteriol. 2017 12 01; 199(23). View in: PubMed

  38. Kant S, Asthana S, Missiakas D, Pancholi V. A novel STK1-targeted small-molecule as an "antibiotic resistance breaker" against multidrug-resistant Staphylococcus aureus. Sci Rep. 2017 07 11; 7(1):5067. View in: PubMed

  39. Missiakas D, Schneewind O. Assembly and Function of the Bacillus anthracis S-Layer. Annu Rev Microbiol. 2017 09 08; 71:79-98. View in: PubMed

  40. Claes J, Liesenborghs L, Peetermans M, Veloso TR, Missiakas D, Schneewind O, Mancini S, Entenza JM, Hoylaerts MF, Heying R, Verhamme P, Vanassche T. Clumping factor A, von Willebrand factor-binding protein and von Willebrand factor anchor Staphylococcus aureus to the vessel wall. J Thromb Haemost. 2017 05; 15(5):1009-1019. View in: PubMed

  41. Yu W, Kim HK, Rauch S, Schneewind O, Missiakas D. Pathogenic conversion of coagulase-negative staphylococci. Microbes Infect. 2017 02; 19(2):101-109. View in: PubMed

  42. Oh SY, Lunderberg JM, Chateau A, Schneewind O, Missiakas D. Genes Required for Bacillus anthracis Secondary Cell Wall Polysaccharide Synthesis. J Bacteriol. 2017 01 01; 199(1). View in: PubMed

  43. Ohr RJ, Anderson M, Shi M, Schneewind O, Missiakas D. EssD, a Nuclease Effector of the Staphylococcus aureus ESS Pathway. J Bacteriol. 2017 01 01; 199(1). View in: PubMed

  44. Anderson M, Ohr RJ, Aly KA, Nocadello S, Kim HK, Schneewind CE, Schneewind O, Missiakas D. EssE Promotes Staphylococcus aureus ESS-Dependent Protein Secretion To Modify Host Immune Responses during Infection. J Bacteriol. 2017 01 01; 199(1). View in: PubMed

  45. Missiakas D, Schneewind O. Staphylococcus aureus vaccines: Deviating from the carol. J Exp Med. 2016 08 22; 213(9):1645-53. View in: PubMed

  46. Malachowa N, Kobayashi SD, Porter AR, Braughton KR, Scott DP, Gardner DJ, Missiakas DM, Schneewind O, DeLeo FR. Contribution of Staphylococcus aureus Coagulases and Clumping Factor A to Abscess Formation in a Rabbit Model of Skin and Soft Tissue Infection. PLoS One. 2016; 11(6):e0158293. View in: PubMed

  47. Kim HK, Falugi F, Missiakas DM, Schneewind O. Peptidoglycan-linked protein A promotes T cell-dependent antibody expansion during Staphylococcus aureus infection. Proc Natl Acad Sci U S A. 2016 May 17; 113(20):5718-23. View in: PubMed

  48. Thomer L, Schneewind O, Missiakas D. Pathogenesis of Staphylococcus aureus Bloodstream Infections. Annu Rev Pathol. 2016 05 23; 11:343-64. View in: PubMed

  49. Thomer L, Emolo C, Thammavongsa V, Kim HK, McAdow ME, Yu W, Kieffer M, Schneewind O, Missiakas D. Antibodies against a secreted product of Staphylococcus aureus trigger phagocytic killing. J Exp Med. 2016 Mar 07; 213(3):293-301. View in: PubMed

  50. Chan YG, Frankel MB, Missiakas D, Schneewind O. SagB Glucosaminidase Is a Determinant of Staphylococcus aureus Glycan Chain Length, Antibiotic Susceptibility, and Protein Secretion. J Bacteriol. 2016 Jan 25; 198(7):1123-36. View in: PubMed

  51. Liszewski Zilla M, Lunderberg JM, Schneewind O, Missiakas D. Bacillus anthracis lcp Genes Support Vegetative Growth, Envelope Assembly, and Spore Formation. J Bacteriol. 2015 Dec; 197(23):3731-41. View in: PubMed

  52. Lunderberg JM, Liszewski Zilla M, Missiakas D, Schneewind O. Bacillus anthracis tagO Is Required for Vegetative Growth and Secondary Cell Wall Polysaccharide Synthesis. J Bacteriol. 2015 Nov; 197(22):3511-20. View in: PubMed

  53. Thammavongsa V, Kim HK, Missiakas D, Schneewind O. Staphylococcal manipulation of host immune responses. Nat Rev Microbiol. 2015 Sep; 13(9):529-43. View in: PubMed

  54. Nguyen-Mau SM, Oh SY, Schneewind DI, Missiakas D, Schneewind O. Bacillus anthracis SlaQ Promotes S-Layer Protein Assembly. J Bacteriol. 2015 Oct; 197(19):3216-27. View in: PubMed

  55. Fan Y, Tan K, Chhor G, Butler EK, Jedrzejczak RP, Missiakas D, Joachimiak A. EsxB, a secreted protein from Bacillus anthracis forms two distinct helical bundles. Protein Sci. 2015 Sep; 24(9):1389-400. View in: PubMed

  56. Oh SY, Richter SG, Missiakas DM, Schneewind O. Glutamate Racemase Mutants of Bacillus anthracis. J Bacteriol. 2015 Jun; 197(11):1854-61. View in: PubMed

  57. Kim HK, Falugi F, Thomer L, Missiakas DM, Schneewind O. Protein A suppresses immune responses during Staphylococcus aureus bloodstream infection in guinea pigs. mBio. 2015 Jan 06; 6(1). View in: PubMed

  58. Thammavongsa V, Rauch S, Kim HK, Missiakas DM, Schneewind O. Protein A-neutralizing monoclonal antibody protects neonatal mice against Staphylococcus aureus. Vaccine. 2015 Jan 15; 33(4):523-6. View in: PubMed

  59. Liszewski Zilla M, Chan YG, Lunderberg JM, Schneewind O, Missiakas D. LytR-CpsA-Psr enzymes as determinants of Bacillus anthracis secondary cell wall polysaccharide assembly. J Bacteriol. 2015 Jan; 197(2):343-53. View in: PubMed

  60. Rauch S, Gough P, Kim HK, Schneewind O, Missiakas D. Vaccine protection of leukopenic mice against Staphylococcus aureus bloodstream infection. Infect Immun. 2014 Nov; 82(11):4889-98. View in: PubMed

  61. Claes J, Vanassche T, Peetermans M, Liesenborghs L, Vandenbriele C, Vanhoorelbeke K, Missiakas D, Schneewind O, Hoylaerts MF, Heying R, Verhamme P. Adhesion of Staphylococcus aureus to the vessel wall under flow is mediated by von Willebrand factor-binding protein. Blood. 2014 Sep 04; 124(10):1669-76. View in: PubMed

  62. Wang YT, Missiakas D, Schneewind O. GneZ, a UDP-GlcNAc 2-epimerase, is required for S-layer assembly and vegetative growth of Bacillus anthracis. J Bacteriol. 2014 Aug 15; 196(16):2969-78. View in: PubMed

  63. Kim HK, Missiakas D, Schneewind O. Mouse models for infectious diseases caused by Staphylococcus aureus. J Immunol Methods. 2014 Aug; 410:88-99. View in: PubMed

  64. Chan YG, Kim HK, Schneewind O, Missiakas D. The capsular polysaccharide of Staphylococcus aureus is attached to peptidoglycan by the LytR-CpsA-Psr (LCP) family of enzymes. J Biol Chem. 2014 May 30; 289(22):15680-90. View in: PubMed

  65. Schneewind O, Missiakas D. Genetic manipulation of Staphylococcus aureus. Curr Protoc Microbiol. 2014 Feb 06; 32:Unit 9C.3.. View in: PubMed

  66. Becker S, Frankel MB, Schneewind O, Missiakas D. Release of protein A from the cell wall of Staphylococcus aureus. Proc Natl Acad Sci U S A. 2014 Jan 28; 111(4):1574-9. View in: PubMed

  67. Schneewind O, Missiakas D. Lipoteichoic acids, phosphate-containing polymers in the envelope of gram-positive bacteria. J Bacteriol. 2014 Mar; 196(6):1133-42. View in: PubMed

  68. Cheng AG, Missiakas D, Schneewind O. The giant protein Ebh is a determinant of Staphylococcus aureus cell size and complement resistance. J Bacteriol. 2014 Mar; 196(5):971-81. View in: PubMed

  69. Thomer L, Becker S, Emolo C, Quach A, Kim HK, Rauch S, Anderson M, Leblanc JF, Schneewind O, Faull KF, Missiakas D. N-acetylglucosaminylation of serine-aspartate repeat proteins promotes Staphylococcus aureus bloodstream infection. J Biol Chem. 2014 Feb 07; 289(6):3478-86. View in: PubMed

  70. Kim HK, Emolo C, Missiakas D, Schneewind O. A monoclonal antibody that recognizes the E domain of staphylococcal protein A. Vaccine. 2014 Jan 16; 32(4):464-9. View in: PubMed

  71. Schneewind O, Missiakas D. Sec-secretion and sortase-mediated anchoring of proteins in Gram-positive bacteria. Biochim Biophys Acta. 2014 Aug; 1843(8):1687-97. View in: PubMed

  72. Thammavongsa V, Missiakas DM, Schneewind O. Staphylococcus aureus degrades neutrophil extracellular traps to promote immune cell death. Science. 2013 Nov 15; 342(6160):863-6. View in: PubMed

  73. Anderson M, Aly KA, Chen YH, Missiakas D. Secretion of atypical protein substrates by the ESAT-6 secretion system of Staphylococcus aureus. Mol Microbiol. 2013 Nov; 90(4):734-43. View in: PubMed

  74. Falugi F, Kim HK, Missiakas DM, Schneewind O. Role of protein A in the evasion of host adaptive immune responses by Staphylococcus aureus. mBio. 2013 Aug 27; 4(5):e00575-13. View in: PubMed

  75. Thomer L, Schneewind O, Missiakas D. Multiple ligands of von Willebrand factor-binding protein (vWbp) promote Staphylococcus aureus clot formation in human plasma. J Biol Chem. 2013 Sep 27; 288(39):28283-92. View in: PubMed

  76. Chan YG, Frankel MB, Dengler V, Schneewind O, Missiakas D. Staphylococcus aureus mutants lacking the LytR-CpsA-Psr family of enzymes release cell wall teichoic acids into the extracellular medium. J Bacteriol. 2013 Oct; 195(20):4650-9. View in: PubMed

  77. Vanassche T, Peetermans M, Van Aelst LN, Peetermans WE, Verhaegen J, Missiakas DM, Schneewind O, Hoylaerts MF, Verhamme P. The role of staphylothrombin-mediated fibrin deposition in catheter-related Staphylococcus aureus infections. J Infect Dis. 2013 Jul; 208(1):92-100. View in: PubMed

  78. Missiakas DM, Schneewind O. Growth and laboratory maintenance of Staphylococcus aureus. Curr Protoc Microbiol. 2013 Feb; Chapter 9:Unit 9C.1. View in: PubMed

  79. Richter SG, Elli D, Kim HK, Hendrickx AP, Sorg JA, Schneewind O, Missiakas D. Small molecule inhibitor of lipoteichoic acid synthesis is an antibiotic for Gram-positive bacteria. Proc Natl Acad Sci U S A. 2013 Feb 26; 110(9):3531-6. View in: PubMed

  80. Oh SY, Maier H, Schroeder J, Richter GS, Elli D, Musser JM, Quenee LE, Missiakas DM, Schneewind O. Vaccine protection against Bacillus cereus-mediated respiratory anthrax-like disease in mice. Infect Immun. 2013 Mar; 81(3):1008-17. View in: PubMed

  81. Lunderberg JM, Nguyen-Mau SM, Richter GS, Wang YT, Dworkin J, Missiakas DM, Schneewind O. Bacillus anthracis acetyltransferases PatA1 and PatA2 modify the secondary cell wall polysaccharide and affect the assembly of S-layer proteins. J Bacteriol. 2013 Mar; 195(5):977-89. View in: PubMed

  82. Chen YH, Anderson M, Hendrickx AP, Missiakas D. Characterization of EssB, a protein required for secretion of ESAT-6 like proteins in Staphylococcus aureus. BMC Microbiol. 2012 Sep 25; 12:219. View in: PubMed

  83. Kim HK, Emolo C, DeDent AC, Falugi F, Missiakas DM, Schneewind O. Protein A-specific monoclonal antibodies and prevention of Staphylococcus aureus disease in mice. Infect Immun. 2012 Oct; 80(10):3460-70. View in: PubMed

  84. McAdow M, DeDent AC, Emolo C, Cheng AG, Kreiswirth BN, Missiakas DM, Schneewind O. Coagulases as determinants of protective immune responses against Staphylococcus aureus. Infect Immun. 2012 Oct; 80(10):3389-98. View in: PubMed

  85. Rauch S, DeDent AC, Kim HK, Bubeck Wardenburg J, Missiakas DM, Schneewind O. Abscess formation and alpha-hemolysin induced toxicity in a mouse model of Staphylococcus aureus peritoneal infection. Infect Immun. 2012 Oct; 80(10):3721-32. View in: PubMed

  86. Garufi G, Hendrickx AP, Beeri K, Kern JW, Sharma A, Richter SG, Schneewind O, Missiakas D. Synthesis of lipoteichoic acids in Bacillus anthracis. J Bacteriol. 2012 Aug; 194(16):4312-21. View in: PubMed

  87. Kern VJ, Kern JW, Theriot JA, Schneewind O, Missiakas D. Surface-layer (S-layer) proteins sap and EA1 govern the binding of the S-layer-associated protein BslO at the cell septa of Bacillus anthracis. J Bacteriol. 2012 Aug; 194(15):3833-40. View in: PubMed

  88. Nguyen-Mau SM, Oh SY, Kern VJ, Missiakas DM, Schneewind O. Secretion genes as determinants of Bacillus anthracis chain length. J Bacteriol. 2012 Aug; 194(15):3841-50. View in: PubMed

  89. Garufi G, Wang YT, Oh SY, Maier H, Missiakas DM, Schneewind O. Sortase-conjugation generates a capsule vaccine that protects guinea pigs against Bacillus anthracis. Vaccine. 2012 May 14; 30(23):3435-44. View in: PubMed

  90. Schneewind O, Missiakas DM. Protein secretion and surface display in Gram-positive bacteria. Philos Trans R Soc Lond B Biol Sci. 2012 Apr 19; 367(1592):1123-39. View in: PubMed

  91. Deng X, Sun F, Ji Q, Liang H, Missiakas D, Lan L, He C. Expression of multidrug resistance efflux pump gene norA is iron responsive in Staphylococcus aureus. J Bacteriol. 2012 Apr; 194(7):1753-62. View in: PubMed

  92. McAdow M, Missiakas DM, Schneewind O. Staphylococcus aureus secretes coagulase and von Willebrand factor binding protein to modify the coagulation cascade and establish host infections. J Innate Immun. 2012; 4(2):141-8. View in: PubMed

  93. DeDent A, Kim HK, Missiakas D, Schneewind O. Exploring Staphylococcus aureus pathways to disease for vaccine development. Semin Immunopathol. 2012 Mar; 34(2):317-33. View in: PubMed

  94. Kim HK, Thammavongsa V, Schneewind O, Missiakas D. Recurrent infections and immune evasion strategies of Staphylococcus aureus. Curr Opin Microbiol. 2012 Feb; 15(1):92-9. View in: PubMed

  95. Thammavongsa V, Schneewind O, Missiakas DM. Enzymatic properties of Staphylococcus aureus adenosine synthase (AdsA). BMC Biochem. 2011 Oct 28; 12:56. View in: PubMed

  96. McAdow M, Kim HK, Dedent AC, Hendrickx AP, Schneewind O, Missiakas DM. Preventing Staphylococcus aureus sepsis through the inhibition of its agglutination in blood. PLoS Pathog. 2011 Oct; 7(10):e1002307. View in: PubMed

  97. Frankel MB, Hendrickx AP, Missiakas DM, Schneewind O. LytN, a murein hydrolase in the cross-wall compartment of Staphylococcus aureus, is involved in proper bacterial growth and envelope assembly. J Biol Chem. 2011 Sep 16; 286(37):32593-605. View in: PubMed

  98. Kim HK, Kim HY, Schneewind O, Missiakas D. Identifying protective antigens of Staphylococcus aureus, a pathogen that suppresses host immune responses. FASEB J. 2011 Oct; 25(10):3605-12. View in: PubMed

  99. Schneewind O, Missiakas D. Structural vaccinology to thwart antigenic variation in microbial pathogens. Proc Natl Acad Sci U S A. 2011 Jun 21; 108(25):10029-30. View in: PubMed

  100. Anderson VJ, Kern JW, McCool JW, Schneewind O, Missiakas D. The SLH-domain protein BslO is a determinant of Bacillus anthracis chain length. Mol Microbiol. 2011 Jul; 81(1):192-205. View in: PubMed

  101. Cheng AG, DeDent AC, Schneewind O, Missiakas D. A play in four acts: Staphylococcus aureus abscess formation. Trends Microbiol. 2011 May; 19(5):225-32. View in: PubMed

  102. Anderson M, Chen YH, Butler EK, Missiakas DM. EsaD, a secretion factor for the Ess pathway in Staphylococcus aureus. J Bacteriol. 2011 Apr; 193(7):1583-9. View in: PubMed

  103. Frankel MB, Wojcik BM, DeDent AC, Missiakas DM, Schneewind O. ABI domain-containing proteins contribute to surface protein display and cell division in Staphylococcus aureus. Mol Microbiol. 2010 Oct; 78(1):238-52. View in: PubMed

  104. Kim HK, Cheng AG, Kim HY, Missiakas DM, Schneewind O. Nontoxigenic protein A vaccine for methicillin-resistant Staphylococcus aureus infections in mice. J Exp Med. 2010 Aug 30; 207(9):1863-70. View in: PubMed

  105. Cheng AG, McAdow M, Kim HK, Bae T, Missiakas DM, Schneewind O. Contribution of coagulases towards Staphylococcus aureus disease and protective immunity. PLoS Pathog. 2010 Aug 05; 6(8):e1001036. View in: PubMed

  106. Lan L, Cheng A, Dunman PM, Missiakas D, He C. Golden pigment production and virulence gene expression are affected by metabolisms in Staphylococcus aureus. J Bacteriol. 2010 Jun; 192(12):3068-77. View in: PubMed

  107. Kim HK, DeDent A, Cheng AG, McAdow M, Bagnoli F, Missiakas DM, Schneewind O. IsdA and IsdB antibodies protect mice against Staphylococcus aureus abscess formation and lethal challenge. Vaccine. 2010 Aug 31; 28(38):6382-92. View in: PubMed

  108. Thammavongsa V, Kern JW, Missiakas DM, Schneewind O. Staphylococcus aureus synthesizes adenosine to escape host immune responses. J Exp Med. 2009 Oct 26; 206(11):2417-27. View in: PubMed

  109. Wu R, Richter S, Zhang RG, Anderson VJ, Missiakas D, Joachimiak A. Crystal structure of Bacillus anthracis transpeptidase enzyme CapD. J Biol Chem. 2009 Sep 04; 284(36):24406-14. View in: PubMed

  110. Cheng AG, Kim HK, Burts ML, Krausz T, Schneewind O, Missiakas DM. Genetic requirements for Staphylococcus aureus abscess formation and persistence in host tissues. FASEB J. 2009 Oct; 23(10):3393-404. View in: PubMed

  111. Richter S, Anderson VJ, Garufi G, Lu L, Budzik JM, Joachimiak A, He C, Schneewind O, Missiakas D. Capsule anchoring in Bacillus anthracis occurs by a transpeptidation reaction that is inhibited by capsidin. Mol Microbiol. 2009 Jan; 71(2):404-20. View in: PubMed

  112. Chen PR, Nishida S, Poor CB, Cheng A, Bae T, Kuechenmeister L, Dunman PM, Missiakas D, He C. A new oxidative sensing and regulation pathway mediated by the MgrA homologue SarZ in Staphylococcus aureus. Mol Microbiol. 2009 Jan; 71(1):198-211. View in: PubMed

  113. DeDent A, Bae T, Missiakas DM, Schneewind O. Signal peptides direct surface proteins to two distinct envelope locations of Staphylococcus aureus. EMBO J. 2008 Oct 22; 27(20):2656-68. View in: PubMed

  114. Garufi G, Butler E, Missiakas D. ESAT-6-like protein secretion in Bacillus anthracis. J Bacteriol. 2008 Nov; 190(21):7004-11. View in: PubMed

  115. Burts ML, DeDent AC, Missiakas DM. EsaC substrate for the ESAT-6 secretion pathway and its role in persistent infections of Staphylococcus aureus. Mol Microbiol. 2008 Aug; 69(3):736-46. View in: PubMed

  116. Bae T, Glass EM, Schneewind O, Missiakas D. Generating a collection of insertion mutations in the Staphylococcus aureus genome using bursa aurealis. Methods Mol Biol. 2008; 416:103-16. View in: PubMed

  117. Maresso AW, Wu R, Kern JW, Zhang R, Janik D, Missiakas DM, Duban ME, Joachimiak A, Schneewind O. Activation of inhibitors by sortase triggers irreversible modification of the active site. J Biol Chem. 2007 Aug 10; 282(32):23129-39. View in: PubMed

  118. Bubeck Wardenburg J, Williams WA, Missiakas D. Host defenses against Staphylococcus aureus infection require recognition of bacterial lipoproteins. Proc Natl Acad Sci U S A. 2006 Sep 12; 103(37):13831-6. View in: PubMed

  119. Gründling A, Missiakas DM, Schneewind O. Staphylococcus aureus mutants with increased lysostaphin resistance. J Bacteriol. 2006 Sep; 188(17):6286-97. View in: PubMed

  120. Blaylock B, Riordan KE, Missiakas DM, Schneewind O. Characterization of the Yersinia enterocolitica type III secretion ATPase YscN and its regulator, YscL. J Bacteriol. 2006 May; 188(10):3525-34. View in: PubMed

  121. Tam C, Glass EM, Anderson DM, Missiakas D. Transposon mutagenesis of Bacillus anthracis strain Sterne using Bursa aurealis. Plasmid. 2006 Jul; 56(1):74-7. View in: PubMed

  122. Tam C, Missiakas D. Changes in lipopolysaccharide structure induce the sigma(E)-dependent response of Escherichia coli. Mol Microbiol. 2005 Mar; 55(5):1403-12. View in: PubMed

  123. Burts ML, Williams WA, DeBord K, Missiakas DM. EsxA and EsxB are secreted by an ESAT-6-like system that is required for the pathogenesis of Staphylococcus aureus infections. Proc Natl Acad Sci U S A. 2005 Jan 25; 102(4):1169-74. View in: PubMed

  124. Williams WA, Zhang RG, Zhou M, Joachimiak G, Gornicki P, Missiakas D, Joachimiak A. The membrane-associated lipoprotein-9 GmpC from Staphylococcus aureus binds the dipeptide GlyMet via side chain interactions. Biochemistry. 2004 Dec 28; 43(51):16193-202. View in: PubMed

  125. Bae T, Banger AK, Wallace A, Glass EM, Aslund F, Schneewind O, Missiakas DM. Staphylococcus aureus virulence genes identified by bursa aurealis mutagenesis and nematode killing. Proc Natl Acad Sci U S A. 2004 Aug 17; 101(33):12312-7. View in: PubMed

  126. Rybin V, Zapun A, Törrönen A, Raina S, Missiakas D, Creighton TE, Metcalf P. Crystallization of DsbC, the disulfide bond isomerase of Escherichia coli. Acta Crystallogr D Biol Crystallogr. 1996 Nov 01; 52(Pt 6):1219-21. View in: PubMed

  127. Zhang R, Wu R, Joachimiak G, Mazmanian SK, Missiakas DM, Gornicki P, Schneewind O, Joachimiak A. Structures of sortase B from Staphylococcus aureus and Bacillus anthracis reveal catalytic amino acid triad in the active site. Structure. 2004 Jul; 12(7):1147-56. View in: PubMed

  128. Mazmanian SK, Skaar EP, Gaspar AH, Humayun M, Gornicki P, Jelenska J, Joachmiak A, Missiakas DM, Schneewind O. Passage of heme-iron across the envelope of Staphylococcus aureus. Science. 2003 Feb 07; 299(5608):906-9. View in: PubMed

  129. Goulding CW, Sawaya MR, Parseghian A, Lim V, Eisenberg D, Missiakas D. Thiol-disulfide exchange in an immunoglobulin-like fold: structure of the N-terminal domain of DsbD. Biochemistry. 2002 Jun 04; 41(22):6920-7. View in: PubMed

  130. Tam C, Collinet B, Lau G, Raina S, Missiakas D. Interaction of the conserved region 4.2 of sigma(E) with the RseA anti-sigma factor. J Biol Chem. 2002 Jul 26; 277(30):27282-7. View in: PubMed

  131. Collinet B, Yuzawa H, Chen T, Herrera C, Missiakas D. RseB binding to the periplasmic domain of RseA modulates the RseA:sigmaE interaction in the cytoplasm and the availability of sigmaE.RNA polymerase. J Biol Chem. 2000 Oct 27; 275(43):33898-904. View in: PubMed

  132. Dartigalongue C, Missiakas D, Raina S. Characterization of the Escherichia coli sigma E regulon. J Biol Chem. 2001 Jun 15; 276(24):20866-75. View in: PubMed

  133. Krupp R, Chan C, Missiakas D. DsbD-catalyzed transport of electrons across the membrane of Escherichia coli. J Biol Chem. 2001 Feb 02; 276(5):3696-701. View in: PubMed

  134. Chung J, Chen T, Missiakas D. Transfer of electrons across the cytoplasmic membrane by DsbD, a membrane protein involved in thiol-disulphide exchange and protein folding in the bacterial periplasm. Mol Microbiol. 2000 Mar; 35(5):1099-109. View in: PubMed

  135. Missiakas D, Raina S. The extracytoplasmic function sigma factors: role and regulation. Mol Microbiol. 1998 Jun; 28(6):1059-66. View in: PubMed

  136. van Straaten M, Missiakas D, Raina S, Darby NJ. The functional properties of DsbG, a thiol-disulfide oxidoreductase from the periplasm of Escherichia coli. FEBS Lett. 1998 May 29; 428(3):255-8. View in: PubMed

  137. Andersen CL, Matthey-Dupraz A, Missiakas D, Raina S. A new Escherichia coli gene, dsbG, encodes a periplasmic protein involved in disulphide bond formation, required for recycling DsbA/DsbB and DsbC redox proteins. Mol Microbiol. 1997 Oct; 26(1):121-32. View in: PubMed

  138. Raina S, Missiakas D. Making and breaking disulfide bonds. Annu Rev Microbiol. 1997; 51:179-202. View in: PubMed

  139. Missiakas D, Mayer MP, Lemaire M, Georgopoulos C, Raina S. Modulation of the Escherichia coli sigmaE (RpoE) heat-shock transcription-factor activity by the RseA, RseB and RseC proteins. Mol Microbiol. 1997 Apr; 24(2):355-71. View in: PubMed

  140. Missiakas D, Raina S. Signal transduction pathways in response to protein misfolding in the extracytoplasmic compartments of E. coli: role of two new phosphoprotein phosphatases PrpA and PrpB. EMBO J. 1997 Apr 01; 16(7):1670-85. View in: PubMed

  141. Missiakas D, Raina S. Protein folding in the bacterial periplasm. J Bacteriol. 1997 Apr; 179(8):2465-71. View in: PubMed

  142. Missiakas D, Raina S. Protein misfolding in the cell envelope of Escherichia coli: new signaling pathways. Trends Biochem Sci. 1997 Feb; 22(2):59-63. View in: PubMed

  143. Missiakas D, Schwager F, Betton JM, Georgopoulos C, Raina S. Identification and characterization of HsIV HsIU (ClpQ ClpY) proteins involved in overall proteolysis of misfolded proteins in Escherichia coli. EMBO J. 1996 Dec 16; 15(24):6899-909. View in: PubMed

  144. Metheringham R, Tyson KL, Crooke H, Missiakas D, Raina S, Cole JA. Effects of mutations in genes for proteins involved in disulphide bond formation in the periplasm on the activities of anaerobically induced electron transfer chains in Escherichia coli K12. Mol Gen Genet. 1996 Nov 27; 253(1-2):95-102. View in: PubMed

  145. Missiakas D, Betton JM, Raina S. New components of protein folding in extracytoplasmic compartments of Escherichia coli SurA, FkpA and Skp/OmpH. Mol Microbiol. 1996 Aug; 21(4):871-84. View in: PubMed

  146. Betton JM, Boscus D, Missiakas D, Raina S, Hofnung M. Probing the structural role of an alpha beta loop of maltose-binding protein by mutagenesis: heat-shock induction by loop variants of the maltose-binding protein that form periplasmic inclusion bodies. J Mol Biol. 1996 Sep 20; 262(2):140-50. View in: PubMed

  147. Missiakas D, Georgopoulos C, Raina S. The Escherichia coli heat shock gene htpY: mutational analysis, cloning, sequencing, and transcriptional regulation. J Bacteriol. 1993 May; 175(9):2613-24. View in: PubMed

  148. Missiakas D, Georgopoulos C, Raina S. The Escherichia coli dsbC (xprA) gene encodes a periplasmic protein involved in disulfide bond formation. EMBO J. 1994 Apr 15; 13(8):2013-20. View in: PubMed

  149. Raina S, Missiakas D, Baird L, Kumar S, Georgopoulos C. Identification and transcriptional analysis of the Escherichia coli htrE operon which is homologous to pap and related pilin operons. J Bacteriol. 1993 Aug; 175(16):5009-21. View in: PubMed

  150. Raina S, Missiakas D, Georgopoulos C. The rpoE gene encoding the sigma E (sigma 24) heat shock sigma factor of Escherichia coli. EMBO J. 1995 Mar 01; 14(5):1043-55. View in: PubMed

  151. Missiakas D, Georgopoulos C, Raina S. Identification and characterization of the Escherichia coli gene dsbB, whose product is involved in the formation of disulfide bonds in vivo. Proc Natl Acad Sci U S A. 1993 Aug 01; 90(15):7084-8. View in: PubMed

  152. Missiakas D, Schwager F, Raina S. Identification and characterization of a new disulfide isomerase-like protein (DsbD) in Escherichia coli. EMBO J. 1995 Jul 17; 14(14):3415-24. View in: PubMed

  153. Zapun A, Missiakas D, Raina S, Creighton TE. Structural and functional characterization of DsbC, a protein involved in disulfide bond formation in Escherichia coli. Biochemistry. 1995 Apr 18; 34(15):5075-89. View in: PubMed

  154. Yon JM, Betton JM, Desmadril M, Mitraki A, Minard P, Gaillard S, Ballery N, Missiakas D. Survey of the folding pathway of a two-domain protein phosphoglycerate kinase. J Chromatogr. 1988 May 25; 440:421-37. View in: PubMed

  155. Minard P, Hall L, Betton JM, Missiakas D, Yon JM. Efficient expression and characterization of isolated structural domains of yeast phosphoglycerate kinase generated by site-directed mutagenesis. Protein Eng. 1989 Oct; 3(1):55-60. View in: PubMed

  156. Fairbrother WJ, Minard P, Hall L, Betton JM, Missiakas D, Yon JM, Williams RJ. Nuclear magnetic resonance studies of isolated structural domains of yeast phosphoglycerate kinase. Protein Eng. 1989 Oct; 3(1):5-11. View in: PubMed

  157. Missiakas D, Betton JM, Minard P, Yon JM. Unfolding-refolding of the domains in yeast phosphoglycerate kinase: comparison with the isolated engineered domains. Biochemistry. 1990 Sep 18; 29(37):8683-9. View in: PubMed

  158. Yon JM, Desmadril M, Betton JM, Minard P, Ballery N, Missiakas D, Gaillard-Miran S, Perahia D, Mouawad L. Flexibility and folding of phosphoglycerate kinase. Biochimie. 1990 Jun-Jul; 72(6-7):417-29. View in: PubMed

  159. Betton JM, Missiakas D, Yon JM. The slow-refolding step of phosphoglycerate kinase as monitored by pulse proteolysis. Arch Biochem Biophys. 1992 Jul; 296(1):95-101. View in: PubMed

  160. Missiakas D, Betton JM, Chaffotte A, Minard P, Yon JM. Kinetic studies of the refolding of yeast phosphoglycerate kinase: comparison with the isolated engineered domains. Protein Sci. 1992 Nov; 1(11):1485-93. View in: PubMed