Lucia Rothman-Denes

  • School of Sciences, University of Buenos Aires, Buenos Aires, Argentina, Licenciado Chemistry 1964
  • School of Sciences, University of Buenos Aires, Buenos Aires, Argentina, PhD Biochemistry 1967
  • NIAMD, NIH, Bethesda, MD, USA, none Molecular Biology 1970
  • University of Chicago, Chicago, IL, USA, none Virology 1974
Awards & Honors
  • 1988 - Award for excellence in graduate teaching University of Chicago
  • 1992 - elected fellow American Academy of Microbiology
  • 2000 - elected fellow American Association for the Advancement of Science
  • 2001 - elected member American Academy of Arts and Sciences
  • 2014 - elected member National Academy of Sciences, USA
  1. Lenneman BR, Rothman-Denes LB. Structural and biochemical investigation of bacteriophage N4-encoded RNA polymerases. Biomolecules. 2015 Apr 27; 5(2):647-67. View in: PubMed

  2. Rothman-Denes LB. Structure of Escherichia coli RNA polymerase holoenzyme at last. Proc Natl Acad Sci U S A. 2013 Dec 03; 110(49):19662-3. View in: PubMed

  3. Gleghorn ML, Davydova EK, Basu R, Rothman-Denes LB, Murakami KS. X-ray crystal structures elucidate the nucleotidyl transfer reaction of transcript initiation using two nucleotides. Proc Natl Acad Sci U S A. 2011 Mar 01; 108(9):3566-71. View in: PubMed

  4. Yano ST, Rothman-Denes LB. A phage-encoded inhibitor of Escherichia coli DNA replication targets the DNA polymerase clamp loader. Mol Microbiol. 2011 Mar; 79(5):1325-38. View in: PubMed

  5. Gleghorn ML, Davydova EK, Rothman-Denes LB, Murakami KS. Structural basis for DNA-hairpin promoter recognition by the bacteriophage N4 virion RNA polymerase. Mol Cell. 2008 Dec 05; 32(5):707-17. View in: PubMed

  6. Davydova EK, Kaganman I, Kazmierczak KM, Rothman-Denes LB. Identification of bacteriophage N4 virion RNA polymerase-nucleic acid interactions in transcription complexes. J Biol Chem. 2009 Jan 23; 284(4):1962-70. View in: PubMed

  7. McPartland J, Rothman-Denes LB. The tail sheath of bacteriophage N4 interacts with the Escherichia coli receptor. J Bacteriol. 2009 Jan; 191(2):525-32. View in: PubMed

  8. Choi KH, McPartland J, Kaganman I, Bowman VD, Rothman-Denes LB, Rossmann MG. Insight into DNA and protein transport in double-stranded DNA viruses: the structure of bacteriophage N4. J Mol Biol. 2008 May 02; 378(3):726-36. View in: PubMed

  9. Murakami KS, Davydova EK, Rothman-Denes LB. X-ray crystal structure of the polymerase domain of the bacteriophage N4 virion RNA polymerase. Proc Natl Acad Sci U S A. 2008 Apr 01; 105(13):5046-51. View in: PubMed

  10. Rothman-Denes LB, Parsot C. Cell regulation. Curr Opin Microbiol. 2008 Apr; 11(2):75-7. View in: PubMed

  11. Rothman-Denes L. Transposons. Science. 1982 Jan 01; 215(4528):52-3. View in: PubMed

  12. Davydova EK, Santangelo TJ, Rothman-Denes LB. Bacteriophage N4 virion RNA polymerase interaction with its promoter DNA hairpin. Proc Natl Acad Sci U S A. 2007 Apr 24; 104(17):7033-8. View in: PubMed

  13. Stojkovic EA, Rothman-Denes LB. Coliphage N4 N-acetylmuramidase defines a new family of murein hydrolases. J Mol Biol. 2007 Feb 16; 366(2):406-19. View in: PubMed

  14. Altman S, Bassler BL, Beckwith J, Belfort M, Berg HC, Bloom B, Brenchley JE, Campbell A, Collier RJ, Connell N, Cozzarelli NR, Craig NL, Darst S, Ebright RH, Elledge SJ, Falkow S, Galan JE, Gottesman M, Gourse R, Grindley ND, Gross CA, Grossman A, Hochschild A, Howe M, Hurwitz J, Isberg RR, Kaplan S, Kornberg A, Kustu SG, Landick RC, Landy A, Levy SB, Losick R, Long SR, Maloy SR, Mekalanos JJ, Neidhardt FC, Pace NR, Ptashne M, Roberts JW, Roth JR, Rothman-Denes LB, Salyers A, Schaechter M, Shapiro L, Silhavy TJ, Simon MI, Walker G, Yanofsky C, Zinder N. An open letter to Elias Zerhouni. Science. 2005 Mar 04; 307(5714):1409-10. View in: PubMed

  15. Davydova EK, Kazmierczak KM, Rothman-Denes LB. Bacteriophage N4-coded, virion-encapsulated DNA-dependent RNA polymerase. Methods Enzymol. 2003; 370:83-94. View in: PubMed

  16. Carter RH, Demidenko AA, Hattingh-Willis S, Rothman-Denes LB. Phage N4 RNA polymerase II recruitment to DNA by a single-stranded DNA-binding protein. Genes Dev. 2003 Sep 15; 17(18):2334-45. View in: PubMed

  17. Davydova EK, Rothman-Denes LB. Escherichia coli single-stranded DNA-binding protein mediates template recycling during transcription by bacteriophage N4 virion RNA polymerase. Proc Natl Acad Sci U S A. 2003 Aug 05; 100(16):9250-5. View in: PubMed

  18. Kazmierczak KM, Davydova EK, Mustaev AA, Rothman-Denes LB. The phage N4 virion RNA polymerase catalytic domain is related to single-subunit RNA polymerases. EMBO J. 2002 Nov 01; 21(21):5815-23. View in: PubMed

  19. Willis SH, Kazmierczak KM, Carter RH, Rothman-Denes LB. N4 RNA polymerase II, a heterodimeric RNA polymerase with homology to the single-subunit family of RNA polymerases. J Bacteriol. 2002 Sep; 184(18):4952-61. View in: PubMed

  20. Van Dyk TK, Wei Y, Hanafey MK, Dolan M, Reeve MJ, Rafalski JA, Rothman-Denes LB, LaRossa RA. A genomic approach to gene fusion technology. Proc Natl Acad Sci U S A. 2001 Feb 27; 98(5):2555-60. View in: PubMed

  21. Rothman-Denes LB, Dai X, Davydova E, Carter R, Kazmierczak K. Transcriptional regulation by DNA structural transitions and single-stranded DNA-binding proteins. Cold Spring Harb Symp Quant Biol. 1998; 63:63-73. View in: PubMed

  22. Dai X, Rothman-Denes LB. DNA structure and transcription. Curr Opin Microbiol. 1999 Apr; 2(2):126-30. View in: PubMed

  23. Dai X, Kloster M, Rothman-Denes LB. Sequence-dependent extrusion of a small DNA hairpin at the N4 virion RNA polymerase promoters. J Mol Biol. 1998; 283(1):43-58. View in: PubMed

  24. Dai X, Rothman-Denes LB. Sequence and DNA structural determinants of N4 virion RNA polymerase-promoter recognition. Genes Dev. 1998 Sep 01; 12(17):2782-90. View in: PubMed

  25. Dai X, Greizerstein MB, Nadas-Chinni K, Rothman-Denes LB. Supercoil-induced extrusion of a regulatory DNA hairpin. Proc Natl Acad Sci U S A. 1997 Mar 18; 94(6):2174-9. View in: PubMed

  26. Miller A, Wood D, Ebright RH, Rothman-Denes LB. RNA polymerase beta' subunit: a target of DNA binding-independent activation. Science. 1997 Mar 14; 275(5306):1655-7. View in: PubMed

  27. Miller A, Dai X, Choi M, Glucksmann-Kuis MA, Rothman-Denes LB. Single-stranded DNA-binding proteins as transcriptional activators. Methods Enzymol. 1996; 274:9-20. View in: PubMed

  28. Glucksmann-Kuis MA, Dai X, Markiewicz P, Rothman-Denes LB. E. coli SSB activates N4 virion RNA polymerase promoters by stabilizing a DNA hairpin required for promoter recognition. Cell. 1996 Jan 12; 84(1):147-54. View in: PubMed

  29. Kiino DR, Singer MS, Rothman-Denes LB. Two overlapping genes encoding membrane proteins required for bacteriophage N4 adsorption. J Bacteriol. 1993 Nov; 175(21):7081-5. View in: PubMed

  30. Kiino DR, Licudine R, Wilt K, Yang DH, Rothman-Denes LB. A cytoplasmic protein, NfrC, is required for bacteriophage N4 adsorption. J Bacteriol. 1993 Nov; 175(21):7074-80. View in: PubMed

  31. Cho NY, Choi M, Rothman-Denes LB. The bacteriophage N4-coded single-stranded DNA-binding protein (N4SSB) is the transcriptional activator of Escherichia coli RNA polymerase at N4 late promoters. J Mol Biol. 1995 Mar 03; 246(4):461-71. View in: PubMed

  32. Choi M, Miller A, Cho NY, Rothman-Denes LB. Identification, cloning, and characterization of the bacteriophage N4 gene encoding the single-stranded DNA-binding protein. A protein required for phage replication, recombination, and late transcription. J Biol Chem. 1995 Sep 22; 270(38):22541-7. View in: PubMed

  33. Falco SC, Zehring W, Rothman-Denes LB. DNA-dependent RNA polymerase from bacteriophage N4 virions. Purification and characterization. J Biol Chem. 1980 May 10; 255(9):4339-47. View in: PubMed

  34. Zehring WA, Rothman-Denes LB. Purification and characterization of coliphage N4 RNA polymerase II activity from infected cell extracts. J Biol Chem. 1983 Jul 10; 258(13):8074-80. View in: PubMed

  35. Zehring WA, Falco SC, Malone C, Rothman-Denes LB. Bacteriophage N4-induced transcribing activities in E. coli. III. A third cistron required for N4 RNA polymerase II activity. Virology. 1983 Apr 30; 126(2):678-87. View in: PubMed

  36. Zivin R, Zehring W, Rothman-Denes LB. Transcriptional map of bacteriophage N4. Location and polarity of N4 RNAs. J Mol Biol. 1981 Oct 25; 152(2):335-56. View in: PubMed

  37. Zivin R, Malone C, Rothman-Denes LB. Physical map of coliphage N4 DNA. Virology. 1980 Jul 15; 104(1):205-18. View in: PubMed

  38. Rothman-Denes LB, Cabib E. Glucose 6-phosphate dependent and independent forms of yeast glycogen synthetase. Their properties and interconversions. Biochemistry. 1971 Mar 30; 10(7):1236-42. View in: PubMed

  39. Rothman-Denes LB, Cabib E. Two forms of yeast glycogen synthetase and their role in glycogen accumulation. Proc Natl Acad Sci U S A. 1970 Jul; 66(3):967-74. View in: PubMed

  40. Rothman-Denes L, Martin RG. Two mutations in the first gene of the histidine operon of Salmonella typhimurium affecting control. J Bacteriol. 1971 Apr; 106(1):227-37. View in: PubMed

  41. Rothman-Denes LB, Schito GC. Novel transcribing activities in N4-infected Escherichia coli. Virology. 1974 Jul; 60(1):65-72. View in: PubMed

  42. Haselkorn R, Rothman-Denes LB. Protein synthesis. Annu Rev Biochem. 1973; 42:397-438. View in: PubMed

  43. Rothman-Denes LB, Hesse JE, Epstein W. Role of cyclic adenosine 3',5'-monophosphate in the in vivo expression of the galactose operon of Escherichia coli. J Bacteriol. 1973 Jun; 114(3):1040-4. View in: PubMed

  44. Rothman-Denes LB, Haselkorn R, Schito GC. Selective shutoff of catabolite-sensitive host syntheses by bacroxyurea pharmaco mutation genes coliphages growth l virus replication escherichia coli growth l lysogeny crosses genetic coliteriophage N4. Virology. 1972 Oct; 50(1):95-102. View in: PubMed

  45. Cabib E, Rothman-Denes LB, Huang K. The regulation of glycogen synthesis in yeast. Ann N Y Acad Sci. 1973 Feb 09; 210:192-206. View in: PubMed

  46. Haynes LL, Rothman-Denes LB. N4 virion RNA polymerase sites of transcription initiation. Cell. 1985 Jun; 41(2):597-605. View in: PubMed

  47. Guinta D, Lindberg G, Rothman-Denes LB. Bacteriophage N4-coded 5'----3' exonuclease. Purification and characterization. J Biol Chem. 1986 Aug 15; 261(23):10736-43. View in: PubMed

  48. Rist JK, Pearle M, Sugino A, Rothman-Denes LB. Development of an in vitro bacteriophage N4 DNA replication system. J Biol Chem. 1986 Aug 15; 261(23):10506-10. View in: PubMed

  49. Lindberg GK, Rist JK, Kunkel TA, Sugino A, Rothman-Denes LB. Purification and characterization of bacteriophage N4-induced DNA polymerase. J Biol Chem. 1988 Aug 15; 263(23):11319-26. View in: PubMed

  50. Markiewicz P, Glucksmann A, Rothman-Denes LB. N4 virion DNA dependent-RNA polymerase: initiation sequences utilized by the enzyme on heterologous templates. Nucleic Acids Res. 1988 Feb 11; 16(3):1011-26. View in: PubMed

  51. Malone C, Spellman S, Hyman D, Rothman-Denes LB. Cloning and generation of a genetic map of bacteriophage N4 DNA. Virology. 1988 Feb; 162(2):328-36. View in: PubMed

  52. Ohmori H, Haynes LL, Rothman-Denes LB. Structure of the ends of the coliphage N4 genome. J Mol Biol. 1988 Jul 05; 202(1):1-10. View in: PubMed

  53. Hartmann GR, Biebricher C, Glaser SJ, Grosse F, Katzameyer MJ, Lindner AJ, Mosig H, Nasheuer HP, Rothman-Denes LB, Schäffner AR, et al. Twelfth Fritz Lipmann lecture. Initiation of transcription--a general tool for affinity labeling of RNA polymerases by autocatalysis. Biol Chem Hoppe Seyler. 1988 Sep; 369(9):775-88. View in: PubMed

  54. Guinta D, Stambouly J, Falco SC, Rist JK, Rothman-Denes LB. Host and phage-coded functions required for coliphage N4 DNA replication. Virology. 1986 Apr 15; 150(1):33-44. View in: PubMed

  55. Kiino DR, Rothman-Denes LB. Genetic analysis of bacteriophage N4 adsorption. J Bacteriol. 1989 Sep; 171(9):4595-602. View in: PubMed

  56. Lindberg G, Kowalczykowski SC, Rist JK, Sugino A, Rothman-Denes LB. Purification and characterization of the coliphage N4-coded single-stranded DNA binding protein. J Biol Chem. 1989 Jul 25; 264(21):12700-8. View in: PubMed

  57. Abravaya K, Rothman-Denes LB. In vitro requirements for N4 RNA polymerase II-specific initiation. J Biol Chem. 1989 Jul 25; 264(21):12695-9. View in: PubMed

  58. Muglia L, Rothman-Denes LB. Cell type-specific negative regulatory element in the control region of the rat alpha-fetoprotein gene. Proc Natl Acad Sci U S A. 1986 Oct; 83(20):7653-7. View in: PubMed

  59. Abravaya K, Rothman-Denes LB. N4 RNA polymerase II sites of transcription initiation. J Mol Biol. 1990 Jan 20; 211(2):359-72. View in: PubMed

  60. Glucksmann MA, Markiewicz P, Malone C, Rothman-Denes LB. Specific sequences and a hairpin structure in the template strand are required for N4 virion RNA polymerase promoter recognition. Cell. 1992 Aug 07; 70(3):491-500. View in: PubMed

  61. Markiewicz P, Malone C, Chase JW, Rothman-Denes LB. Escherichia coli single-stranded DNA-binding protein is a supercoiled template-dependent transcriptional activator of N4 virion RNA polymerase. Genes Dev. 1992 Oct; 6(10):2010-9. View in: PubMed

  62. Falco SC, Rothman-Denes LB. Bacteriophage N4-induced transcribing activities in Escherichia coli. II. Association of the N4 transcriptional apparatus with the cytoplasmic membrane. Virology. 1979 Jun; 95(2):466-75. View in: PubMed

  63. Falco SC, Rothman-Denes LB. Bacteriophage N4-induced transcribing activities in Escherichia coli. I. Detection and characterization in cell extracts. Virology. 1979 Jun; 95(2):454-65. View in: PubMed

  64. Falco SC, Zivin R, Rothman-Denes LB. Novel template requirements of N4 virion RNA polymerase. Proc Natl Acad Sci U S A. 1978 Jul; 75(7):3220-4. View in: PubMed

  65. Falco SC, Laan KV, Rothman-Denes LB. Virion-associated RNA polymerase required for bacteriophage N4 development. Proc Natl Acad Sci U S A. 1977 Feb; 74(2):520-3. View in: PubMed

  66. vander Laan K, Falco SC, Rothman-Denes LB. The program of RNA synthesis in N4-infected Escherichia coli. Virology. 1977 Feb; 76(2):596-601. View in: PubMed

  67. Hesse JE, Rothman-Denes LB, Epstein W. A convenient erythrocyte membrane cyclic AMP binding assay. Anal Biochem. 1975 Sep; 68(1):202-8. View in: PubMed

  68. Epstein W, Rothman-Denes LB, Hesse J. Adenosine 3':5'-cyclic monophosphate as mediator of catabolite repression in Escherichia coli. Proc Natl Acad Sci U S A. 1975 Jun; 72(6):2300-4. View in: PubMed